塘栖产枇杷叶的药材品质分析

发布时间:2019-08-30 来源: 短文摘抄 点击:


  [摘要] 目的 评估塘栖产不同品种枇杷叶的药材品质。 方法 参照2015版《中国药典》,采用烘干法、灼烧法、热浸法分析测定塘栖产不同品种枇杷叶的水分、总灰分和浸出物含量;采用Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-甲醇-0.5%醋酸铵(67∶12∶21,v/v/v)为流动相,柱温25℃,流速为1.0 mL/min,检测波长为210 nm,对齐墩果酸和熊果酸的含量进行测定。采用Kromasil C18(250 mm×4.6 mm,5 μm)色谱柱,以甲醇-0.4%磷酸水溶液(50∶50,v/v)为流动相,柱温30℃,流速为1.0 mL/min,检测波长为360 nm,对槲皮素和山奈酚的含量进行测定。结果 塘栖产各品种枇杷叶水分、总灰分、浸出物含量范围分别为7.87%~9.45%、5.14%~6.22%、25.41%~29.68%;齐墩果酸和熊果酸的含量总和范围为1.04%~1.25%,均符合2015版《中国药典》的标准;此外,枇杷叶中的槲皮素和山奈酚的总含量范围为0.17%~0.29%。 结论 塘栖产地的不同品种枇杷叶均具有较好的品质,值得进一步开发利用。
  [关键词] 枇杷叶;齐墩果酸;熊果酸;总黄酮
  [中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210(2018)10(c)-0111-04
  [Abstract] Objective To evaluate the quality of different varieties of loquat leaves from Tangxi. Methods Reference to the 2015 edition of Chinese Pharmacopoeia, the water content, total ash content and extract content of different varieties of loquat leaves were measured by oven drying method, ignition method and hot-dip method. The oleanolic acid and ursolic acid were detected useing Kromasil C18 (250 mm×4.6 mm, 5 μm) column, with mobile phase being acetonitrile-methanol-0.5% ammonium acetate (67∶12∶21, v/v/v), column temperature being at 25℃, flow velocity being 1.0 mL/min, and detection wavelength being at 210 nm. The quercetin and kaempferol were detected by Kromasil C18(250 mm×4.6 mm,5 μm)column, with mobile phase being methanol-0.4% phosphoric acid (50∶50, v/v), column temperature being at 30℃, flow velocity being 1.0 mL/min, and detection wavelength being at 360 nm. Results The range of water content, total ash content, and extract content of different varieties of loquat leaves were 7.87%-9.45%, 5.14%-6.22% and 25.41%-29.68%, respectively, the range of total content of oleanolic acid and ursolic acid was 1.04%-1.25%, all in line with the 2015 edition of Chinese Pharmacopoeia. In addition, the range of total content of quercetin and kaempferol in loquat leaves was 0.17%-0.29%. Conclusion The different varieties of loquat leaves in Tangxi all have good quality and deserve further development and utilization.
  [Key words] Loquat leaf; Oleanolic acid; Ursolic acid; Total flavonoid
  枇杷葉是一味临床常用传统中药,来源于蔷薇科植物枇杷Eriobotrya japonica(Thunb.)Lindl.的干燥叶片,收载于历版《中国药典》[1]中,性微寒,味苦辛,归肺、胃经,具有清肺止咳、降逆止呕等功效,是目前中医常用药,临床使用量大。现代药理研究表明,枇杷叶中的化学成分结构多样,主要有三萜酸类、黄酮类、倍半萜、多酚类、糖苷类以及挥发油类化合物[2-7]。近年来研究表明枇杷叶除具有较好的抗炎和止咳作用外,还具有其他广泛的药理作用,如降血糖、抗肿瘤、抗病毒、保肝和抗氧化等[2,8-17]。通过对枇杷叶化学成分和药理作用的研究,可以看出枇杷叶这一传统中药具有多方面的用途,可用于多种疾病的预防和治疗,并取得了良好的疗效。
  我国枇杷资源丰富,主要分布于浙江、江西、广东、福建、台湾、四川、贵州、云南、陕西、甘肃、江苏、安徽等地。其中杭州市余杭区塘栖镇是全国著名的枇杷产区之一,枇杷栽种历史悠久,有“中国枇杷之乡”的美誉。检索文献发现,虽然关于枇杷叶的相关研究报道很多,但目前还没有关于塘栖枇杷叶品质的文献报道,对塘栖这一主要枇杷产地枇杷叶系统研究尚未进行过,这与其果实品质不相称。本研究对塘栖产不同种源枇杷叶的水分、总灰分、浸出物、齐墩果酸、熊果酸和总黄酮含量进行了分析测定,旨在为塘栖枇杷叶资源利用提供科学依据。现报道如下:

相关热词搜索:药材 品质 分析 枇杷叶 塘栖产

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